ELISpot Plus: Human TNF‑α (ALP)

Human TNF-α ELISpot. PBMC incubated in the presence or absence of PHA.

ELISpot Plus: Human TNF‑α (ALP)

3512-4APW-2

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This offer is valid when purchasing ELISpot Plus: Human TNF-α (ALP) or other qualifying products.

Publications: 20

Documents

Bulk or custom format

The easiest way to achieve reproducibility in ELISpot.

Components

Plate	Pre-coated ELISpot plate, white MSIP (mAb MT25C5)
Detection mAb	MT20D9, biotin
Enzyme conjugate	Streptavidin-ALP
Substrate	BCIP/NBT-plus

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Delivery 4-9 business days

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Complementary products

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PepPool: CEFTA (CD4), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human TNF-α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Human TNF-α (ALP)
Application	ELISpot
Analyte	TNF-α
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human TNF-α.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

Documents

Datasheet/Protocol SDS

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Publications (20)

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Analyte information

TNF-α

Analyte description	Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.
Alternative names	Tumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell type	T cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID	7124