ELISpot Plus: Human TNF‑α (ALP)

Human TNF-α ELISpot. PBMC incubated in the presence or absence of PHA.
Human TNF-α ELISpot. PBMC incubated in the presence or absence of PHA.

ELISpot Plus: Human TNF‑α (ALP)

3512-4APW-2

Special offer

You may add one these complementary products at a reduced price.

PepPool: CEFTA (CD4), human
  • Reactivity:
  • Application:
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PepPool: CEF (CD8), human
  • Reactivity:
  • Application:
  • Plates:

This offer is valid when purchasing ELISpot Plus: Human TNF-α (ALP) or other qualifying products.

Publications: 20
Documents
Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT25C5)
Detection mAbMT20D9, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

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Delivery 4-9 business days

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Complementary products
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PepPool: CEFTA (CD4), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human TNF-α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Human TNF-α (ALP)
ApplicationELISpot
AnalyteTNF-α
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human TNF-α.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
Human TNF-α ELISpot. PBMC incubated in the presence or absence of PHA.
Human TNF-α ELISpot. PBMC incubated in the presence or absence of PHA.
Human TNF-α ELISpot. PBMC incubated in the presence or absence of PHA.
Datasheet/ProtocolSDS

Highlighted research
Published: October 11, 2024 | 8 minute read

Immunoassays for vaccine research

Explore the critical role of immunoassays like ELISpot and FluoroSpot in vaccine development, from evaluating immune responses to tracking vaccine efficacy and potency. These assays provide sensitive, single-cell insights for advancing research and development of viral, bacterial, and cancer vaccines.

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID7124
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FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α