ELISpot Plus: Human TNF‑α (ALP)

Human TNF-α ELISpot. PBMC incubated in the presence or absence of PHA.

ELISpot Plus: Human TNF‑α (ALP)

3512-4APW-2

Special offer

You may add any of these complementary products at a reduced price.

PepPool: CEFRAS Global (CD4), human

Reactivity:
Application:
Plates:

PepPool: CEFRAS Global (CD4 and CD8), human

Reactivity:
Application:
Plates:

PepPool: CEFRAS Global (CD8), human

Reactivity:
Application:
Plates:

This offer is valid when purchasing ELISpot Plus: Human TNF-α (ALP) or other qualifying products.

Publications: 21

Documents

Bulk or custom format

The easiest way to achieve reproducibility in ELISpot.

Components

Plate	Pre-coated ELISpot plate, white MSIP (mAb MT25C5)
Detection mAb	MT20D9, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot
Substrate	BCIP/NBT-plus substrate for ELISpot

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

1 / 5

PepPool: CEF (CD8), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human TNF-α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Human TNF-α (ALP)
Application	ELISpot
Analyte	TNF-α
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human TNF-α.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

Documents

Datasheet/Protocol SDS

Product highlights

Featured product
Published: January 21, 2025 | 7 minute read

New positive control peptide pools: CEFRAS Global

Discover the PepPool: CEFRAS Global family of peptide pools delivering superior T cell stimulation across diverse populations. With meticulously chosen epitopes from a number of common pathogens, it sets a new standard for ELISpot, FluoroSpot, and flow cytometry.

Highlighted research
Published: October 11, 2024 | 8 minute read

Immunoassays for vaccine research

Explore the critical role of immunoassays like ELISpot and FluoroSpot in vaccine development, from evaluating immune responses to tracking vaccine efficacy and potency. These assays provide sensitive, single-cell insights for advancing research and development of viral, bacterial, and cancer vaccines.

Tutorials

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (21)

Loading publications...

Analyte information

TNF-α

Analyte description	Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.
Alternative names	Tumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell type	T cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID	7124