FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α

FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α

FSP-010209-2

Ensure reproducibility when diving into a multiplex analysis.

Components

Detection mAbsAnti-IFN-y mAb (7-B6-1), BAM
Anti-IL-2 mAb (MT8G10), biotin
Anti-TNF-α mAb (MT20D9), WASP
Fluorophore conjugatesAnti-BAM mAb, 490
SA-550
Anti-WASP mAb, 640
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)
Buffer/SolutionFluoroSpot enhancer
PlatePre-coated FluoroSpot plate (mAbs 1-D1K, MT2A91/2C95, and MT25C5)

In stock

Delivery 3-7 days

Shipping $0.00

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ, IL-2, and TNF-α using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductFluoroSpot Plus: Human IFN-γ/IL-2/TNF-α
ApplicationFluoroSpot
AnalyteIFN-γ, IL-2, TNF-α
ReactivityHuman
Specificity

This kit is based on matched pairs of mAbs specific for human IFN-γ, IL-2, and TNF-α.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Human PBMC (250,000 cells/well) were stimulated with the CEF peptide pool and anti-CD28 antibody (both available from Mabtech) overnight and the number of cells secreting IFN-γ, IL-2 and TNF-α were analyzed by FluoroSpot using Mabtech IRIS.