|Detection mAbs||Anti-IFN-γ mAb (7-B6-1), BAM|
|Anti-IL-2 mAb (MT8G10), biotin|
|Anti-TNF-α mAb (MT20D9), WASP|
|Fluorophore conjugates||Anti-BAM mAb, 490|
|Anti-WASP mAb, 640|
|Positive control||Anti-CD3 mAb (CD3-2)|
|Co-stimulus||Anti-CD28 mAb (CD28-A)|
|Plate||Pre-coated FluoroSpot plate (mAbs 1-D1K, MT2A91/2C95, and MT25C5)|
Delivery 4-9 business days
This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ, IL-2, and TNF-α using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.
|Product||FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α|
|Analyte||IFN-γ, IL-2, TNF-α|
This kit is based on matched pairs of mAbs specific for human IFN-γ, IL-2, and TNF-α.
Shipping and Storage
Shipped at ambient temperature.
Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
|Shelf life||At least 12 months from date of receipt.|
Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
|Alternative names||Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69|
|Cell type||T cell, Tc, Th1, NK cell|
Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases.
|Alternative names||Interleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF, lymphokine|
|Cell type||T cell, Th1, Th2, Th17, Tfh|
Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.
|Alternative names||Tumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F|
|Cell type||T cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ|
Highlighted research, Published May 17, 2022
Highlighted research, Published December 16, 2021
Highlighted research, Published December 2, 2021
Highlighted research, Published November 24, 2021