FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α

FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α

FSP-010209-2

Publications: 3
Documents
Questions?
Ensure reproducibility when diving into a multiplex analysis.

Components

Detection mAbsAnti-IFN-y mAb (7-B6-1), BAM
Anti-IL-2 mAb (MT8G10), biotin
Anti-TNF-α mAb (MT20D9), WASP
Fluorophore conjugatesAnti-BAM mAb, 490
SA-550
Anti-WASP mAb, 640
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)
Buffer/SolutionFluoroSpot enhancer
PlatePre-coated FluoroSpot plate (mAbs 1-D1K, MT2A91/2C95, and MT25C5)

In stock

Delivery 4-9 business days

Shipping $0.00

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ, IL-2, and TNF-α using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductFluoroSpot Plus: Human IFN-γ/IL-2/TNF-α
ApplicationFluoroSpot
AnalyteIFN-γ, IL-2, TNF-α
ReactivityHuman
Specificity

This kit is based on matched pairs of mAbs specific for human IFN-γ, IL-2, and TNF-α.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Human PBMC (250,000 cells/well) were stimulated with the CEF peptide pool and anti-CD28 antibody (both available from Mabtech) overnight and the number of cells secreting IFN-γ, IL-2 and TNF-α were analyzed by FluoroSpot using Mabtech IRIS.
Datasheet/ProtocolSDS
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Tutorial, Published October 10, 2022

Step-by-step guide to FluoroSpot
An in-depth guide to FluoroSpot, including best practice and tricks we've learnt over the years.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Highlighted research, Published May 17, 2022

T cell responses against Omicron
Mabtech IRIS and FluoroSpot Flex: Human IFN-γ/TNF-α/IL-2/Granzyme B to assess T cell immunity in mRNA vaccine recipients. Three doses mRNA vaccine had better T cell responses than recipients of two doses.

Highlighted research, Published December 16, 2021

SARS-CoV-2-specific immune responses
SARS-CoV-2 specific immune responses in autoimmunity with Mabtech FluoroSpot, ELISpot Path, and Mabtech IRIS. Patients with rheumatic diseases showed T cell responses comparable to healthy control. Patients with MS and after mRNA vaccination show different memory B cell IgG responses.

Highlighted research, Published December 2, 2021

SARS-CoV-2-specific T and memory B cells
Detection of SARS-CoV-2-specific T and memory B cells 6 and 15 months after infection. The authors utilized ELISpot Path: SARS-CoV-2 (RBD) Human IgG (ALP) and FluoroSpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ/IL-2. Analysis were performed on Mabtech IRIS.

Highlighted research, Published November 24, 2021

Polyfunctional T cells with Mabtech IRIS
Study of development and persistence of virus-specific cellular and humoral immune responses, during the acute phase and two follow-ups after COVID-19 disease in hospitalized patients. Mabtech’s FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α were analyzed with Mabtech IRIS.

Highlighted research, Published May 29, 2018

Elucidate pleiotropic effects of lenalidomide
Mabtech FluoroSpot Plus: IFN-γ/IL-2/TNF-α was utilized to show the polyfunctional profile of CD8+ T cells in patients with multiple myeloma after lenolidomide treatment.