PepPool: CEFTA (CD4), human

PepPool: CEFTA (CD4), human


Publications: 4
Validated for our assays.


Peptide pool with 35 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This peptide pool is intended for use as a positive control in immunoassays such as ELISpot, FluoroSpot, and flow cytometry assays. It induces cytokine secretion from antigen-specific human CD4 T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The CEFTA peptide pool induces secretion of e.g., IFN-γ, IL-2, IL-5, and IL-13 from human CD4 T cells. It consists of 35 MHC class II-restricted peptides from human CMV, EBV, influenza virus, tetanus toxin, and adenovirus 5. It is designed to stimulate T cells with a broad array of HLA types. The synthetic peptides are >95% pure.

Published HLA types covered by the CEFTA peptide pool:
HLA-DRB1*01, HLA-DRB1*03, HLA-DRB1*04, HLA-DRB1*07, HLA-DRB1*08, HLA-DRB1*11, HLA-DRB1*13, HLA-DRB1*15, HLA-DRB1*16, HLA-DRB1*53, HLA-DPw2, HLA-DPB1*04, HLA-DQw1, HLA-DQw3, HLA-DQB1*06.


Product details

ProductPepPool: CEFTA (CD4), human
ApplicationT cell stimulation, ELISpot, FluoroSpot, Flow cytometry
Supplied in



Peptide pool with 35 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

Shipping and Storage


Shipped at ambient temperature.


Store at -20 °C or below upon receipt. 

Human PBMC stimulated with the CEFTA peptide pool (product code 3617-1) and anti-human CD28 antibody in human IFN-γ/IL-5 FluoroSpot. Analysis was done with Mabtech IRIS.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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