Mabtech IRIS

Mabtech IRIS

Mabtech IRIS™ – Spot analysis reinvented

  • ELISpot and FluoroSpot (up to 4 colors) 
  • Precise spot counting 
  • Fixed focus and self-calibrating XY table for a streamlined workflow
  • Intuitive software for a smooth user experience
  • Customizable presets for recurrent experiments

Product specifications

Intended use

Serum/Plasma samples

Recommendation

Application 

ELISpot1 color
FluoroSpot

4 colors

Recommended fluorophores (excitation/emission)

380 nm/430 nm (DAPI equivalent)

490 nm/510 nm (FITC equivalent)  

550 nm/570 nm (Cy3 equivalent)

640 nm/660 nm (Cy5 equivalent)

 

 

Hardware 

XY tableSelf-calibrating
Light source(s)

FluoroSpot: LED380, LED490, LED550, LED640

ELISpot: White LED ring light

Camera sensor             CMOS sensor with global shutter
Camera lens    Telecentric
Resolution (H x W)2048 x 2048 pixels
Plate types 96-well: IPFL, MSIP, MAIPSWU10
ComputerDesktop PC included

 

 

Software

Mabtech Apex™RAWspot™ technology included
Export formats.raw .jpg .xlsx .pzfx

 

Reading speed

ELISpot:
<2 min/plate

FluoroSpot:

1 color <5 min/plate

2 colors <7 min/plate

3 colors<10 min/plate

4 colors<13 min/plate

Operating systemWindows

 

 

Service

Warranty

1 year, option to prolong

Installation

IQ OQ

Regulation

Compliance with CE, RoHS, REACH, WEEE, FCC, ICES, CFR21 part 11

 

 

Shipping and Storage

 

Streamlined workflow

Mabtech IRIS is pre-calibrated for a smooth workflow. Simply insert the plate, select assay type, and press the read button. The entire signal from every spots is recorded so that adjustments can be made after plate reading – you never have to re-read a plate. In addition, the Mabtech Apex™ software offers intuitive layout features and automatic data export to speed up the time from data acquisition to analysis. 

 

 

 

Precise spot counting  

The software for Mabtech IRIS is based on our patented RAWspot™ technology. The RAWspot algorithm is optimized to find the exact location of the spot center. This refined spot center detection is the basis for accurate spot counting and is essential to identify cells secreting multiple analytes in FluoroSpot. 
Mabtech IRIS also delivers a new dimension of spot data – the relative spot volume (RSV) – as RAWspot extracts a 3-dimensional volume for each spot corresponding to the amount of secreted analyte.

 

 



 

Read now, analyze later

After plate reading, you are free to adjust parameters such as brightness, intensity, contrast, and spot size cut-offs without affecting the original data. As IRIS has already recorded the uncompressed signal straight from the camera sensor, changes can be made afterwards without affecting the original data. 
Find out how Mabtech IRIS meets the requirements of CFR21 part 11 (FDA guidelines on electronic records in clinical investigations) here.

 

 

Minimized variability

Automated reader configuration and optimized presets minimize user-defined input and ensure an objective analysis independent of the user’s level of experience. Plus, the intuitive software facilitates smooth onboarding. Spot analysis has never been easier.