Mabtech IRIS

Mabtech IRIS™ – Spot analysis reinvented

IRIS is a FluoroSpot and ELISpot reader capable of analyzing four analytes simultaneously with unparalleled accuracy and speed. The system revolves around our novel RAWspot™ algorithm, ensuring accurate spot center detection and thus correct counting. Together with the intuitive software Mabtech Apex™, our readers provide a plug-and-play system that simplifies your workflow. Just load the plate, select the assay, and press "read".

ELISpot and FluoroSpot (up to 4 colors)
Precise spot counting
Fixed focus and self-calibrating XY table for a streamlined workflow
Intuitive software for a smooth user experience
Customizable presets for recurrent experiments

Product specifications

Intended use

Serum/Plasma samples

Recommendation

Application

ELISpot

1 color

FluoroSpot

4 colors

Recommended fluorophores (excitation/emission):
380 nm/430 nm (DAPI equivalent)
490 nm/510 nm (FITC equivalent)
550 nm/570 nm (Cy3 equivalent)
640 nm/660 nm (Cy5 equivalent)

Hardware

XY table	Self-calibrating
Light source(s)	FluoroSpot: LED380, LED490, LED550, LED640 ELISpot: White LED ring light
Camera sensor	CMOS sensor with global shutter
Camera lens	Telecentric
Resolution (H x W)	2048 x 2048 pixels
Plate types	96-well: IPFL, MSIP, MAIPSWU
Computer	Desktop PC included

Software

Mabtech Apex™	RAWspot™ technology included
Export formats	.raw .jpg .xlsx .pzfx
Reading speed	ELISpot: <2 min/plate FluoroSpot: 1 color <5 min/plate 2 colors <7 min/plate 3 colors<10 min/plate 4 colors<13 min/plate
Operating system	Windows

Service

Warranty	1 year, option to prolong
Installation	IQ OQ
Regulation	Compliance with CE, RoHS, REACH, WEEE, FCC, ICES, CFR21 part 11

Shipping and Storage

Performance

Streamlined workflow

Mabtech IRIS is pre-calibrated for a smooth workflow. Simply insert the plate, select assay type, and press the read button. The entire signal from every spots is recorded so that adjustments can be made after plate reading – you never have to re-read a plate. In addition, the Mabtech Apex™ software offers intuitive layout features and automatic data export to speed up the time from data acquisition to analysis.

Precise spot counting

The software for Mabtech IRIS is based on our patented RAWspot™ technology. The RAWspot algorithm is optimized to find the exact location of the spot center. This refined spot center detection is the basis for accurate spot counting and is essential to identify cells secreting multiple analytes in FluoroSpot.
Mabtech IRIS also delivers a new dimension of spot data – the relative spot volume (RSV) – as RAWspot extracts a 3-dimensional volume for each spot corresponding to the amount of secreted analyte.

Read now, analyze later

After plate reading, you are free to adjust parameters such as brightness, intensity, contrast, and spot size cut-offs without affecting the original data. As IRIS has already recorded the uncompressed signal straight from the camera sensor, changes can be made afterwards without affecting the original data.
Find out how Mabtech IRIS meets the requirements of CFR21 part 11 (FDA guidelines on electronic records in clinical investigations) here.

Minimized variability

Automated reader configuration and optimized presets minimize user-defined input and ensure an objective analysis independent of the user’s level of experience. Plus, the intuitive software facilitates smooth onboarding. Spot analysis has never been easier.