PepPool: TB (ESAT‑6), scanning

PBMC (250,000 cells/well) from a patient with tuberculosis infection were incubated overnight in the absence or presence of the ESAT-6 scanning pool and the number of cells secreting IFN-γ was analyzed by ELISpot.

PepPool: TB (ESAT‑6), scanning

3624-1

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Intended use

This peptide pool is intended for studying immune responses against TB (ESAT-6) using immunoassays such as ELISpot and FluoroSpot. It induces cytokine secretion from antigen-specific human T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The TB (ESAT-6) scanning pool induces secretion of e.g., IFN-γ from human T cells. It contains 21 peptides from the Mycobacterium tuberculosis early secretory antigenic target 6 kDa (ESAT-6) protein. The peptides are 15-mers overlapping with 11 amino acids, covering the complete protein. The mean purity of the synthetic peptides is 98%.

Peptides included in the peptide pool are listed in the datasheet.

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Product details

ProductPepPool: TB (ESAT-6), scanning
ApplicationT cell stimulation, ELISpot, FluoroSpot
ReactivityHuman
Supplied in

Lyophilized

Contents

Peptide pool with 21 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C or below upon receipt. 

PBMC (250,000 cells/well) from a patient with tuberculosis infection were incubated overnight in the absence or presence of the ESAT-6 scanning pool and the number of cells secreting IFN-γ was analyzed by ELISpot.
PBMC (250,000 cells/well) from a patient with tuberculosis infection were incubated overnight in the absence or presence of the ESAT-6 scanning pool and the number of cells secreting IFN-γ was analyzed by ELISpot.

Datasheet/ProtocolSDS

Highlighted research
Published: October 28, 2024 | 18 minute read

Immunoassays for infectious disease research

Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.

Highlighted research
Published: October 28, 2024 | 3 minute read

ELISpot vs ELISA: Superior sensitivity in TB diagnostics

ELISpot is preferred over ELISA in TB diagnostics due to its higher sensitivity, enabling early detection of TB-specific T cells through single-cell analysis of IFN-γ secretion. ELISpot offers a more accurate immune response assessment, crucial for diagnosing TB in humans and animals.

Highlighted research
Published: December 21, 2023 | 1 minute read

White paper: Tuberculosis diagnosis with spots

Early and precise diagnosis is crucial for effective TB management, and ELISpot is increasingly being used for this purpose. This white paper examines the advantages of ELISpot in TB diagnosis and suggests future antigens and biomarkers that can enhance diagnostic accuracy.

Webinar
Published: November 17, 2023 | 1 minute read

Webinar: Novel insights into TB vaccines and diagnostics

Recording now available!

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PepPool: TB (EspC), scanning