PepPool: CEF (CD8), human

PepPool: CEF (CD8), human


Publications: 40
Validated for our assays.


Peptide pool with 23 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This peptide pool is intended for use as a positive control in immunoassays such as ELISpot, FluoroSpot, and flow cytometry. It induces cytokine secretion from antigen-specific human CD8 T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The CEF peptide pool induces secretion of e.g., IFN-γ, IL-2, and granzyme B from human CD8 T cells. It consists of 23 MHC class I-restricted viral peptides from human CMV, EBV, and influenza virus. It has been shown to induce IFN-γ responses in almost 90% of all Caucasians. The synthetic peptides are >95% pure.

Published HLA types covered by the CEF peptide pool:
HLA-A1, HLA-A2, HLA-A3, HLA-A11, HLA-A24, HLA-A68, HLA-B7, HLA-B8, HLA-B27, HLA-B35, HLA-B44

Peptides included in the CEF peptide pool are listed in the datasheet.


Product details

ProductPepPool: CEF (CD8), human
ApplicationT cell stimulation, ELISpot, FluoroSpot, Flow cytometry
Supplied in



Peptide pool with 23 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

Shipping and Storage


Shipped at ambient temperature.


Store at -20 °C or below upon receipt. 

Human PBMC stimulated with the CEF peptide pool (product code 3616-1) in human IFN-γ ELISpot. Analysis was done with Mabtech IRIS.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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