PepPool: SARS-CoV-2 Omicron BA.1 (S1), scanning

PepPool: SARS‑CoV-2 Omicron BA.1 (S1), scanning


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Validated for our assays.


Peptide pool with 168 peptides, 25 ug of each peptide. Supplied in two vials (lyophilized).

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Product specifications

Intended use

This peptide pool is intended for studying immune responses against the spike protein of SARS-CoV-2 (variant Omicron B.1.1.529, linage BA.1) using immunoassays such as ELISpot and FluoroSpot. It induces cytokine secretion from antigen-specific human T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The SARS-CoV-2 Omicron BA.1 (S1) scanning pool induces secretion of e.g., IFN-γ and IL-2 from human T cells. It contains 168 peptides from the human SARS-CoV-2 virus variant Omicron (B.1.1.529), lineage BA.1. The peptides are 15-mers overlapping with 11 amino acids, covering the S1 domain of the spike protein. The peptide pool contains mutations: A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, and P681H of Omicron BA.1 spike S1.

The peptide pool has been validated using human PBMC from COVID-19 convalescent individuals and vaccinated individuals.

The pool is supplied in two vials: Pool 1: SARS-CoV-2 (Omicron BA.1) S1 peptides 1-84 (84 peptides) and pool 2: SARS-CoV-2 (Omicron BA.1) S1 peptides 85-168 (84 peptides). The mean purity of the synthetic peptides is 80%.

Peptides included in the peptide pool are listed in the datasheet.


Product details

ProductPepPool: SARS-CoV-2 Omicron BA.1 (S1), scanning
ApplicationT cell stimulation, ELISpot, FluoroSpot, Flow cytometry
Supplied in



Peptide pool with 168 peptides, 25 ug of each peptide. Supplied in two vials (lyophilized).

Shipping and Storage


Shipped at ambient temperature.


Store at -20 °C or below upon receipt. 

Human PBMC (250,000 cells/well) from a SARS-CoV-2 convalescent individual were incubated for 48 hours in the absence or presence of SARS-CoV-2 (Omicron BA.1) S1 defined peptide (vials 1+2) and anti-CD28 mAb. The number of cells secreting IFN-γ, IL-2 and both analytes was analyzed by FluoroSpot using Mabtech IRIS.

Highlighted research, Published May 17, 2022

Third vaccine dose important for T cell response against Omicron
Three doses mRNA vaccine had better T cell responses than recipients of two doses. Mabtech IRIS and FluoroSpot Flex: Human IFN-γ/TNF-α/IL-2/Granzyme B were used to assess T cell immunity in mRNA vaccine recipients.
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