PepPool: CMV (CD4 and CD8), human

CMV peptide pool used in Flow cytometry to stimulate IFN-γ production by human CD4+ and CD8+ T cells

Human PBMC stimulated with the CMV peptide pool (product code 3619-1) in human IFN-γ ELISpot. Analysis was done with Mabtech IRIS.

PepPool: CMV (CD4 and CD8), human

3619-1

Publications: 9

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Intended use

This peptide pool is intended for studying immune responses against CMV in immunoassays such as ELISpot, FluoroSpot, and flow cytometry. It induces cytokine secretion from antigen-specific human CD4 and CD8 T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The CMV peptide pool induces secretion of e.g., IFN-γ, IL-2, IL-5, and IL-13 from human CD4 and CD8 T cells. It contains 42 peptides from cytomegalovirus. The antigens represented in the peptide pool are pp50, pp65, IE1, IE2, and envelope glycoprotein B. 28 of the peptides are MHC class I-restricted and 14 are MHC class II-restricted. The synthetic peptides are >95% pure.

HLA types covered by the CMV pool:
HLA class I: HLA-A*01:01, A*02:01, A*02:06, A*03:01, A*11:01, A*23:01, A*24:02, A*24:07, A*26:01, A*30:01, A*33; HLA-B*07:02, B*08:01, B*15:01, B*18:01, B*27, B*35:01, B*35:02, B*35:03, B*35:08, B*35:11, B*38:01, B*38:02, B*40:01, B*40:02, B*40:06, B*41, B*42:01, B*42:02, B*44:02, B*44:03, B*51:01, B*53:01, B*57, B*58; HLA-C*04:01, C*06:02, C*07:02.
HLA class II: HLA-DRB1*01, DRB1*03:01, DRB1*07:01, DRB1*08, DRB1*11:01, DRB1*11:04, DRB1*15:01; HLA-DRB3*01:01 (DR52a), DRB3*02:02 (DR52b), DRB4*01 (DR53), DRB5*01 (DR51); HLA-DQA1*01:02/DQB1*06:02 (DQ6), DQB1*03:01; HLA-DPA1*01:03 with DPB1*01:01, DPB1*02:01, DPB1*03:01, DPB1*14:01, DPB1*20:01.

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Product details

Product	PepPool: CMV (CD4 and CD8), human
Application	T cell stimulation, ELISpot, FluoroSpot, Flow cytometry
Reactivity	Human
Supplied in	Lyophilized
Contents	Peptide pool with 42 peptides, 25 ug of each peptide. Supplied in one vial (lyophilized).

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at -20 °C or below upon receipt.

Human PBMC stimulated with CMV peptide pool (2 ug/ml) and anti-CD28 antibody (0.1 ug/ml) for 16 hours (both available from Mabtech) in the presence of Brefeldin A. In donor 1, CD4+ cells start expressing IFN-γ following CMV peptide pool stimulation whereas donor 2 responds by production of IFN-γ by CD8+ cells. Cells were stained with CD4-PE and CD8-FITC prior to fixation and permeabilization using 4% paraformaldehyde and saponin. IFN-γ was detected using 1D1K-PF647P (Mabtech). The dot plots derive from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.