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3420-2HST-2Human IFN-γ ELISpotPRO (HRP), strips

  • ELISpotPRO kit containing strip-plates pre-coated with monoclonal antibody, enzyme-conjugated monoclonal detection antibody and ELISpot substrate. An empty plate frame is included. Anti-CD3 mAb is included as a positive control for cytokine secretion. Color of the vial tops may vary.
  • PBMC (62,500 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IFN-γ was analyzed by ELISpot.
  • PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of the CEF peptide pool (available from Mabtech, code 3615-1) and the number of cells secreting IFN-γ was analyzed by ELISpot.
  • PBMC (62,500 cells/well) were incubated for 20 h in the absence or presence of Phytohaemagglutinin (PHA) and the number of cells secreting IFN-γ was analyzed by ELISpot.
  • PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of Tuberculin purified protein derivative (PPD) and the number of cells secreting IFN-γ was analyzed by ELISpot.
$480
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ELISpotPRO kit with strip plates for enumeration of cells secreting IFN-γ

The ELISpotPRO kit is Mabtech's most innovative product featuring pre-coated plates and one-step detection. The kit enables enumeration of cells secreting human IFN-γ and contains ELISpot 8-well strip-plates pre-coated with monoclonal antibody, HRP-conjugated antibody for one-step detection and TMB substrate. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.

Pre-coated plates and one-step detection enable a more rapid assay with minimized variability. In addition, strip plates are ideal when running a limited number of wells.

Application:
  • ELISpot
Reactivity:
  • Human
Documentation: Product datasheet
Contents:
  • Enzyme-conjugated detection mAb (7-B6-1-HRP)
  • Positive control (anti-CD3 mAb)
  • Substrate (TMB)
  • 2 Pre-coated clear strip plates (mAb 1-D1K)
Size: 2 plates