Highlighted research

Potency of cockroach allergens evaluated with FluoroSpot

Published: November 4, 2019

Updated: October 13, 2023

Birrueta et al. (Front Immunol, 2019) compared the immunogenicity of different German cockroach extracts on PBMCs from allergic and non-allergic patients.

An IFN-γ/IL-10/IL-5 FluoroSpot assay was central to dissect the variable immune profiles among the donors, as it allowed the researchers to unravel the effect of the various extracts directly on T-cell activity. As hypothesized, the different extracts did not only vary in their composition but also had variable biological effects on T cell responses. Furthermore, the team detected a significantly higher IL-5 response in allergic donors compared to non-allergic donors.

In summary, this is the first study to compare various cockroach extracts and their effect on T cells. These findings have implications for the choice of extracts used in diagnostics and allergen specific immunotherapy.

Explore similar topics

Highlighted research FluoroSpotAllergyPublication