FluoroSpot Plus: Human IFN-γ/Granzyme B/IL-2

FluoroSpot Plus: Human IFN-γ/Granzyme B/IL-2


Publications: 2
Ensure reproducibility when diving into a multiplex analysis.


Detection mAbsAnti-IFN-γ mAb (7-B6-1), BAM
Anti-Granzyme B (MT28), biotin
Anti-IL-2 mAb (MT8G10), WASP
Fluorophore conjugatesAnti-BAM mAb, 490
Anti-WASP mAb, 640
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)
Buffer/SolutionFluoroSpot enhancer
PlatePre-coated FluoroSpot plate (mAbs 1-D1K, MT34B6, and MT2A91/2C95)

In stock

Delivery 4-9 business days

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Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ, granzyme B, and IL-2 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples


Product details

ProductFluoroSpot Plus: Human IFN-γ/Granzyme B/IL-2
AnalyteIFN-γ, Granzyme B, IL-2

This kit is based on matched pairs of mAbs specific for human IFN-γ, granzyme B, and IL-2.

Shipping and Storage


Shipped at ambient temperature.


Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
PBMC (250,000 cells/well) were incubated for 42 hours in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1) and anti-CD28 antibody. The numbers of cells secreting IFN-γ, Granzyme B and IL-2 was analyzed by FluoroSpot. Single cytokine secreting cells as well as double- and triple-secreting cells were determined. Spot center was used to identify spots from double- and triple-secreting cells, visualized in an image overlay of LED490, FITC (IFN-γ) and LED550, Cy3 (Granzyme B) and LED640, Cy5 (IL-2). Spot analysis was made with Mabtech IRIS.

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Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458

Granzyme B

Analyte description

Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.

Alternative namesGranzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell typeT cell, Tc, Th1, NK cell
Gene ID3002


Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF, lymphokine
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID3558
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