ELISpot Plus: Human IL‑22 (ALP)

Human PBMC were incubated for 48 h in the absence (500,000 cells/well), or presence (100,000 cells/well) of anti-CD3 monoclonal antibody (available from Mabtech, code 3610-1-50) and the number of cells secreting IL-22 was analyzed by ELISpot.
Human PBMC (500,000 cells/well) were incubated for 48 h in the absence or presence of Candida albicans extract and the number of cells secreting IL-22 was analyzed by ELISpot.

ELISpot Plus: Human IL‑22 (ALP)

3475-4APW-2

$565

Special offer

You may add any of these complementary products at a reduced price.

PepPool: CEFRAS Global (CD4), human
  • Reactivity:
  • Application:
  • Plates:
PepPool: CEFRAS Global (CD4 and CD8), human
  • Reactivity:
  • Application:
  • Plates:

This offer is valid when purchasing ELISpot Plus: Human IL-22 (ALP) or other qualifying products.

Publications: 12
Documents
Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT12A3)
Detection mAbMT7B27, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
SubstrateBCIP/NBT-plus substrate for ELISpot
Positive controlAnti-CD3 mAb (CD3-2)

Low stock

Delivery within 3-4 weeks

Shipping $0

Complementary products

Complementary products
1 / 3

PepPool: CEFTA (CD4), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-22 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Human IL-22 (ALP)
ApplicationELISpot
AnalyteIL-22
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-22.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Human PBMC were incubated for 48 h in the absence (500,000 cells/well), or presence (100,000 cells/well) of anti-CD3 monoclonal antibody (available from Mabtech, code 3610-1-50) and the number of cells secreting IL-22 was analyzed by ELISpot.
Human PBMC were incubated for 48 h in the absence (500,000 cells/well), or presence (100,000 cells/well) of anti-CD3 monoclonal antibody (available from Mabtech, code 3610-1-50) and the number of cells secreting IL-22 was analyzed by ELISpot.
Human PBMC were incubated for 48 h in the absence (500,000 cells/well), or presence (100,000 cells/well) of anti-CD3 monoclonal antibody (available from Mabtech, code 3610-1-50) and the number of cells secreting IL-22 was analyzed by ELISpot.
Human PBMC (500,000 cells/well) were incubated for 48 h in the absence or presence of Candida albicans extract and the number of cells secreting IL-22 was analyzed by ELISpot.
Datasheet/ProtocolSDS

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.
Loading publications...

IL-22

Analyte description

The cytokine interleukin 22 (IL-22) is mainly produced by activated CD4 T helper 17 (Th17) and T helper 22 (Th22) cells. The IL-22 receptor is expressed on non-immune cells, particularly epithelial cells and keratinocytes, and IL-22 promotes innate immune responses versus bacterial infections in these target cells. In addition, Th22 cells have been shown to play a role in the pathophysiology of several human skin diseases.

Alternative namesInterleukin-22, IL-22, IL22, IL-21, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18
Cell typeT cell, Th1, Th17, Tfh
Gene ID50616
1 / 13

FluoroSpot Plus: Human IFN-γ/IL-22/IL-17A