|Anti-IFN-γ mAb (7-B6-1), BAM
|Anti-IL-22 mAb (MT7B27), biotin
|Anti-IL-17A mAb (MT504), WASP
|Anti-BAM mAb, 490
|Anti-WASP mAb, 640
|Anti-CD3 mAb (CD3-2)
|Anti-CD28 mAb (CD28-A)
|Pre-coated FluoroSpot plate (mAbs 1-D1K, MT12A3, and MT44.6)
Delivery 4-9 business days
This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ, IL-22, and IL-17A using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.
|FluoroSpot Plus: Human IFN-γ/IL-22/IL-17A
|IFN-γ, IL-22, IL-17A
This kit is based on matched pairs of mAbs specific for human IFN-γ, IL-22, and IL-17A.
Shipping and Storage
Shipped at ambient temperature.
Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
|At least 12 months from date of receipt.
Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
|Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
|T cell, Tc, Th1, NK cell
The cytokine interleukin 22 (IL-22) is mainly produced by activated CD4 T helper 17 (Th17) and T helper 22 (Th22) cells. The IL-22 receptor is expressed on non-immune cells, particularly epithelial cells and keratinocytes, and IL-22 promotes innate immune responses versus bacterial infections in these target cells. In addition, Th22 cells have been shown to play a role in the pathophysiology of several human skin diseases.
|Interleukin-22, IL-22, IL22, IL-21, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18
|T cell, Th1, Th17, Tfh
Interleukin 17A (IL-17A) is a potent proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has also been shown to be produced by activated CD8 T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce the expression of cytokines, chemokines, and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production, and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer, and transplant rejection.
|Interleukin 17A, IL-17A, IL17A