ELISpot Flex: Human IL‑2 (ALP)

PBMC (50,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 mAb (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-2 was analyzed by ELISpot.
PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of PPD and the number of cells secreting IL-2 was analyzed by ELISpot.

ELISpot Flex: Human IL‑2 (ALP)

3445-2A

Special offer

You may add one these complementary products at a reduced price.

Anti-human CD3 mAb (CD3-2), azide free
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ELISpot substrate: BCIP/NBT‑plus for ALP
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  • Plates:
PepPool: CEFTA (CD4), human
  • Reactivity:
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  • Plates:
ELISpot plates, white
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  • Plates:

This offer is valid when purchasing ELISpot Flex: Human IL-2 (ALP) or other qualifying products.

Publications: 53
Documents
Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT2A91/2C95
Detection mAbMT8G10, biotin
Enzyme conjugateStreptavidin-ALP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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Anti-human CD3 mAb (CD3-2), azide free

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human IL-2 (ALP)
ApplicationELISpot
AnalyteIL-2
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-2.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
PBMC (50,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 mAb (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-2 was analyzed by ELISpot.
PBMC (50,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 mAb (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-2 was analyzed by ELISpot.
PBMC (50,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 mAb (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-2 was analyzed by ELISpot.
PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of PPD and the number of cells secreting IL-2 was analyzed by ELISpot.
Datasheet/ProtocolSDS

Highlighted research
Published: October 11, 2024 | 8 minute read

Immunoassays for vaccine research

Explore the critical role of immunoassays like ELISpot and FluoroSpot in vaccine development, from evaluating immune responses to tracking vaccine efficacy and potency. These assays provide sensitive, single-cell insights for advancing research and development of viral, bacterial, and cancer vaccines.

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

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Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

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Tutorial, November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF, lymphokine
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID3558
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ELISpot Plus: Human IL-2 (ALP)