ELISpot substrate: BCIP/NBT‑plus for ALP

ELISpot substrate: BCIP/NBT‑plus for ALP

3650-10

Publications: 26
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Intended use

This ready-to-use BCIP/NBT-plus substrate is intended for the development of ELISpot assays using Streptavidin-ALP enzyme conjugates. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Adjust to room temperature before use. Filter the substrate solution through a 0.45 µm filter and add to ELISpot plates after incubation with alkaline phosphatase and subsequent washing. Develop until spots emerge. Stop the colour development by washing extensively in tap water.

Product details

ProductELISpot substrate: BCIP/NBT-plus for ALP
ApplicationELISpot
Contents

BCIP/NBT-plus substrate for ELISpot

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.

Datasheet/ProtocolSDS

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Highlighted research, November 8, 2022

Modified ELISpot to detect viral proteins

Detection of secreted antigens by HIV infected cells and quantification of CD4 T cells with adapted ELISpot viral protein spot assay (VIP-Spot).

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ELISpot conjugate: Streptavidin-ALP