ELISpot Flex: Human IL‑10 (ALP)

PBMC (10,000 cells/well) were incubated for 20 h in the absence or presence of LPS and the number of cells secreting IL-10 was analyzed by ELISpot.

ELISpot Flex: Human IL‑10 (ALP)

3430-2A

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Publications: 59
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Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb9D7
Detection mAb12G8, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot

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Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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ELISpot substrate: BCIP/NBT-plus for ALP

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-10 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human IL-10 (ALP)
ApplicationELISpot
AnalyteIL-10
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-10.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
PBMC (10,000 cells/well) were incubated for 20 h in the absence or presence of LPS and the number of cells secreting IL-10 was analyzed by ELISpot.
PBMC (10,000 cells/well) were incubated for 20 h in the absence or presence of LPS and the number of cells secreting IL-10 was analyzed by ELISpot.
Datasheet/ProtocolSDS

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IL-10

Analyte description

Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.

Alternative namesInterleukin-10, IL-10, IL10, CSIF, GVHDS, IL-10A, TGIF
Cell typeT cell, Treg, Monocyte/MΦ, mDC
Gene ID3586
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ELISpot Plus: Human IL-10 (ALP)