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ELISpot

130 topics

No high-level spots were produced after protein stimulation

Started by Guest Mary2 replies • 92 views

Vacuum Manifold

Started by Guest Michael ELISpot2 replies • 84 views

The Light, fuzzy, small dots in ELISPOT

Started by Guest Rain2 replies • 137 views

ELISPOT results interpretation after counting with IRIS

Started by Guest Vivian2 replies • 139 views

Limit of detection for Human IFN-g/IL-4 FluoroSpot Flex Kit (Catalog X-01A16B-10)

Started by Guest Maria2 replies • 277 views

Question: The spots in 3 wells are blue

Started by mKim2 replies • 307 views

Wells appear “marbled” after developing

Started by Guest Maxi4 replies • 577 views

Frozen, cryo-preserved cells

Started by Guest TKN2 replies • 315 views

preservation of pre-coated plates

Started by Guest manny2 replies • 351 views

Result is negative

Started by Sumu5 replies • 455 views

Treatment and long-term preservation of PVDF plates

Started by Guest manny6 replies • 569 views

Cross-reactivity/ staining of membrane by secondary antibody

Started by Guest Amalie4 replies • 625 views

Validation of ELISpot

Started by Guest Margot2 replies • 405 views

Human pan IgG ELISpot - dark membrane and white spots

Started by Guest Emily3 replies • 536 views

Effector target ratio for low antigen load

Started by Gael2 replies • 285 views

Peptide length for ELISPOT?

Started by Guest Guest Andrew5 replies • 766 views

IFN-gamma Elispot with human PBMC activated by adherent tumor cells

Started by Guest Esen Aral2 replies • 510 views

Question about viability of frozen splenocytes after thawing (ELISpot)

Started by Guest A Le Heron2 replies • 521 views

Odd spots in negative control wells

Started by Guest Tim2 replies • 453 views

Help with antigen-specific ELISpot assay

Started by Guest Federico Ruiz Moreno7 replies • 766 views

How long can a coated plate last in the fridge?

Started by Guest Emily3 replies • 893 views

Question about RPMI and cell volume

Started by Guest Federico Ruiz Moreno2 replies • 569 views

Issues with Elispot

Started by Saswati Emma Mohr lab2 replies • 739 views

Buffer solution used for making coating anti human IgG for B cells ELISpot

Started by Guest Syabila4 replies • 757 views

TMB for HRP SAME FOR ELISA AND ELISPOT

Started by Guest Camila Enriquez2 replies • 993 views

Problems with central membrane white spot and retained blue color

Started by Guest Saswati Bhattacharya4 replies • 891 views

Plate Preparation protocol of using Pre-coated ELISpot clear plate

Started by Guest Syabila6 replies • 596 views

Blurry spots in antigen-specific wells

Started by Guest Mason3 replies • 681 views

IFN-g ELISpot using TMB

Started by Guest Hayan2 replies • 406 views

MICE ELISpot Assay

Started by Guest Ivan Ssali2 replies • 424 views

Shelf life of no pre-coated plates.

Started by Guest Mathilde3 replies • 604 views

TMB used in B cell ELISpot development (3825-2H)

Started by Guest Mason6 replies • 957 views

No clear spots in mouse IFN-γ ELISpot

Started by Guest Rain6 replies • 724 views

change of background color after development(B cell ELISpot)

Started by Guest Eunju Jung2 replies • 648 views

Finding Lot Numbers for kits

Started by Guest Stephen3 replies • 392 views

IFN gamma ELISpot on splenocytes form humanized mice_340-2HST-02 kit

Started by FLAVIA4 replies • 647 views

Purple center area

Started by Jun2 replies • 639 views

how to add stimulants and how many micrograms of each protein is appropriate

Started by Guest TANG2 replies • 536 views

how to add stimulants and how many micrograms of each protein is appropriate

Started by Guest TANG2 replies • 463 views

Question about ASC ELISPOT or FLuorospot for sheep

Started by Analia3 replies • 581 views

Question about the standard curve of ELISA Flex

Started by Guest Liu4 replies • 631 views

Bovine ASC elispot

Started by Guest Analía9 replies • 801 views

About precipitate, membrane staining and negative controls in ELISpot assay

Started by Yaice Sandoval6 replies • 1993 views

High background level and Repeatability

Started by Xian Yang4 replies • 845 views

Rabbit IFN-r ELISPOT-Abnormal dark background

Started by Guest Sherry3 replies • 567 views

'Cells alone'/'no stimuli' wells with high number of spots

Started by Guest Carlos7 replies • 1028 views

Dark blue staining of ELIspot membrane

Started by Guest Ellie2 replies • 617 views

Weird ELISPOT development

Started by Guest Elias4 replies • 1028 views

shelf life of pre-coated plates

Started by Guest Stanislava2 replies • 529 views

Use of FCS for blocking

Started by Guest thomson4 replies • 1100 views

Spots in no stimuli wells when using mouse IFNg splenocytes

Started by Guest Subeena Sood5 replies • 1802 views

too many negative spot of IFNg ELISpot

Started by mKim3 replies • 915 views

Colors of IFNg spot

Started by Guest Tan Trieu Nguyen2 replies • 709 views

Using APCs in ELISpot with CD8+ T cells

Started by Guest Nils2 replies • 718 views

Purple wells when developing Mouse IFN-y ELISPOT plus kit 3321-4AST-2

Started by Guest Rongji2 replies • 1834 views

IFNg ELISPOT: Inhibition of background spots in wells for which the donor is seronegative

Started by Guest José Manuel Marín2 replies • 703 views

Purple spot background

Started by Guest Idania2 replies • 1356 views

High Background in IFNg ELISpot for mouse spleenocytes

Started by Guest Sajib6 replies • 1950 views

Antigen-specific IgG spots by antibody-negative donors

Started by Keisuke5 replies • 725 views

Dark "clumps" in peptide-stimulated samples

Started by Guest Tetiana2 replies • 1066 views

Total mouse IgG ELISPOT - not many spots?

Started by Guest Lucy10 replies • 2053 views

Poorly developed ELISpot membrane

Started by Guest Lena4 replies • 1394 views

HBsAg-specific B cell ELISPOT assay

Started by Guest E.J. Jung9 replies • 1468 views

How long stable is the SFC on plate membrane after air-dry

Started by mKim3 replies • 1200 views

IQ/OQ of Eli Scan Plus reader

Started by mKim3 replies • 1555 views

Blank areas in IFNg ELISpot with human PBMCs

Started by Guest Lena5 replies • 914 views

Scientist

Started by Guest Wendy3 replies • 828 views

No spots for IFN-gamma

Started by Guest Kenneth6 replies • 1120 views

ELISPOT B cell IgG background and no spot

Started by Guest Calméjane4 replies • 931 views

ELISpot cell dulitions

Started by Hugo2 replies • 889 views

ELISpot system suitability

Started by Hugo2 replies • 1035 views

IgG background in antigen coated ELISpot

Started by Guest Hari B2 replies • 777 views

Using Tween20 detergent in Wash Steps of ELISpot

Started by Guest Del2 replies • 2678 views

Antigen-specific B cell ELISPOT

Started by akira13 replies • 1622 views

Hydrated too many ELISpot PLUS plates

Started by Guest David2 replies • 726 views

technical support B-cell IgM

Started by Guest Michelle2 replies • 1142 views

Stimuli for ELISPOT

Started by Guest Alok Tembhurne12 replies • 2117 views

IFN-Gamma ELISPOT No Development?

Started by Guest Eva7 replies • 1856 views

B-cell ELISpot IgG dark background staining

Started by Guest Michelle2 replies • 1012 views

Stimuli for Elispot assay

Started by Guest Heidio4 replies • 1092 views

many spots in negative control

Started by Guest Janou3 replies • 2864 views

Mouse IL-4 ELISpot

Started by Guest Lucy7 replies • 1497 views

No spots with human perforin ELISpot kit

Started by Questions from customers6 replies • 1759 views

How can I wash adherent cells completely?

Started by Questions from customers2 replies • 1793 views

Background Problem with Mouse IFN-gamma ELISPOT assay

Started by Guest Pavel10 replies • 4113 views

Total saturation or poor development?

Started by Guest SoCal Elispot5 replies • 1405 views

Maximum sensitivity in ELISpot?

Started by Questions from customers2 replies • 966 views

ELSIPOT of Human IL-12p70 and IL-23

Started by Guest Sap4 replies • 1242 views

supernatant culture ELISPOT

Started by Guest Gabriela AQ2 replies • 1281 views

Patchy wells?

Started by Guest Lai4 replies • 1408 views

PBMC resting after thawing

Started by Guest Jason4 replies • 3676 views

False positve in IFN gamma ELISpot

Started by Guest Flavia6 replies • 2318 views

Number of T cells per well

Started by Guest Sylvain3 replies • 1500 views

Ethanol pre-treatment of strip-plate?

Started by Questions from customers3 replies • 2337 views

Is serum free medium ok in ELISpot?

Started by Questions from customers2 replies • 3204 views

Increasing spot presence in negative controls

Started by Guest Renee8 replies • 4227 views

B cell ELISPOT, positive control

Started by Guest Eunju, Jung2 replies • 1275 views

positive control reference value

Started by Guest Eunju, Jung4 replies • 1654 views

B cell ELISPOT, blurry spot

Started by Guest Eunju, Jung2 replies • 1922 views

Uneven background in mouse IgG ELISpot

Started by Guest Janna5 replies • 3095 views