ELISpot Plus: Human Granzyme B (ALP)

PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1), and the number of cells secreting Granzyme B was analyzed by ELISpot.

ELISpot Plus: Human Granzyme B (ALP)

3486-4APW-2

Special offer

You may add any of these complementary products at a reduced price.

PepPool: CEFRAS Global (CD8), human

Reactivity:
Application:
Plates:

PepPool: CEFRAS Global (CD4 and CD8), human

Reactivity:
Application:
Plates:

This offer is valid when purchasing ELISpot Plus: Human Granzyme B (ALP) or other qualifying products.

Publications: 62

Documents

Bulk or custom format

The easiest way to achieve reproducibility in ELISpot.

Components

Plate	Pre-coated ELISpot plate, white MSIP (mAb MT28)
Detection mAb	MT8610, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot
Substrate	BCIP/NBT-plus substrate for ELISpot

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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PepPool: CEF (CD8), human

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human granzyme B using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Human Granzyme B (ALP)
Application	ELISpot
Analyte	Granzyme B
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human granzyme B.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1), and the number of cells secreting Granzyme B was analyzed by ELISpot.

Datasheet/Protocol SDS

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Tutorial

What is a positive ELISpot response?

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Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

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Granzyme B

Analyte description	Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.
Alternative names	Granzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3002

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FluoroSpot Plus: Human IFN-γ/Granzyme B