FluoroSpot Plus: Human IFN-γ/Granzyme B

FluoroSpot Plus: Human IFN-γ/Granzyme B

FSP-0136-2

Ensure reproducibility when diving into a multiplex analysis.

Components

Detection mAbsAnti-IFN-y mAb (7-B6-1), BAM
Anti-Granzyme B (MT28), biotin
Fluorophore conjugatesAnti-BAM mAb, 490
SA-550
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)
Buffer/SolutionFluoroSpot enhancer
PlatePre-coated FluoroSpot plate (mAbs 1-D1K and MT34B6)

In stock

Delivery 3-7 days

Shipping $0.00

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ and granzyme B using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductFluoroSpot Plus: Human IFN-γ/Granzyme B
ApplicationFluoroSpot
AnalyteIFN-γ, Granzyme B
ReactivityHuman
Specificity

This kit is based on matched pairs of mAbs specific for human IFN-γ and granzyme B.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1). The number of cells secreting IFN-γ, Granzyme B and both analytes was analyzed by FluoroSpot using Mabtech IRIS.