FluoroSpot Plus: Human IFN‑γ/Granzyme B

PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1). The number of cells secreting IFN-γ, Granzyme B and both analytes was analyzed by FluoroSpot using Mabtech IRIS.

FluoroSpot Plus: Human IFN‑γ/Granzyme B

FSP-0136-2

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Publications: 9
Documents
Bulk or custom format
Ensure reproducibility when diving into a multiplex analysis.

Components

PlatePre-coated FluoroSpot plate (mAbs 1-D1K and MT34B6)
Detection mAbsAnti-IFN-γ mAb (7-B6-1), BAM
Anti-Granzyme B (MT28), biotin
Fluorophore conjugatesAnti-BAM mAb, 490
SA-550
Buffer/SolutionFluoroSpot enhancer
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ and granzyme B using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductFluoroSpot Plus: Human IFN-γ/Granzyme B
ApplicationFluoroSpot
AnalyteIFN-γ, Granzyme B
ReactivityHuman
Specificity

This kit is based on matched pairs of mAbs specific for human IFN-γ and granzyme B.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1). The number of cells secreting IFN-γ, Granzyme B and both analytes was analyzed by FluoroSpot using Mabtech IRIS.
PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1). The number of cells secreting IFN-γ, Granzyme B and both analytes was analyzed by FluoroSpot using Mabtech IRIS.
Datasheet/ProtocolSDS

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Tutorial, September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Highlighted research, May 17, 2022

Third vaccine dose important for T cell response against Omicron

Three doses mRNA vaccine had better T cell responses than recipients of two doses. Mabtech IRIS and FluoroSpot Flex: Human IFN-γ/TNF-α/IL-2/Granzyme B were used to assess T cell immunity in mRNA vaccine recipients.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458

Granzyme B

Analyte description

Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.

Alternative namesGranzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell typeT cell, Tc, Th1, NK cell
Gene ID3002
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FluoroSpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ/Granzyme B