Catalog / Human TNF-α ELISpot kits
Human TNF-α ELISpot kits
Pick the right ELISpot kit for Human TNF-α. Our kits differ in what we put in the box and how you develop the signal – this page explains what those differences mean.
TNF-α ELISpot kits are also available for bovine, monkey, mouse, and porcine.
What differs between these kits
Whether a pre-coated plate is included
Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.
Which detection system
Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.
Supplied without a plate, streptavidin conjugate, enough for 4 plates
With a pre-coated white plate, streptavidin conjugate, 2 plates
With a pre-coated white plate, streptavidin conjugate, 10 plates
About TNF-α
These Human TNF-α ELISpot kits target TNF (Homo sapiens, Gene ID 7124).
Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.
| Also known as | Tumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF |
| Produced by | T cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ |
| Reactivity | Human |
| Gene ID | 7124 |
Find the right kit
Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.
Pre-coated plate included
Or coat your own
Kit detection system
Which enzyme conjugate we supply
Next, choose whether you want a pre-coated plate.
Frequently bought together
What customers usually order alongside these kits.
Related reading
Guides and tutorials covering Human TNF-α and the ELISpot method.
