ELISpot substrate: TMB for HRP

ELISpot substrate: TMB for HRP

3651-10

Publications: 16
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Intended use

This ready-to-use TMB substrate is intended for the development of ELISpot assays using Streptavidin-HRP enzyme conjugates. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Adjust to room temperature before use. Add to ELISpot plates after incubation with horseradish peroxidase and subsequent washing. Develop until spots emerge. Stop the colour development by washing extensively in deionized water (as ions may cause TMB spots to fade).

Product details

ProductELISpot substrate: TMB for HRP
ApplicationELISpot
Contents

Ready-to-use TMB substrate for ELISpot

Shipping and Storage

Shipping

Shipped at ambient temperature.

Shelf lifeAt least 18 months from date of receipt.

Datasheet/ProtocolSDS

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

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ELISA/ELISpot conjugate: Streptavidin-HRP