ELISpot substrate: TMB for HRP

ELISpot substrate: TMB for HRP


Publications: 17
Validated for our assays.


Ready-to-use TMB substrate for ELISpot

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ready-to-use TMB substrate is intended for the development of ELISpot assays using Streptavidin-HRP enzyme conjugates. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples


Adjust to room temperature before use. Add to ELISpot plates after incubation with horseradish peroxidase and subsequent washing. Develop until spots emerge. Stop the colour development by washing extensively in deionized water (as ions may cause TMB spots to fade).

Product details

ProductELISpot substrate: TMB for HRP

Ready-to-use TMB substrate for ELISpot

Shipping and Storage


Shipped at ambient temperature.

Shelf lifeAt least 18 months from date of receipt.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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