Catalog / Human IL-4 ELISpot kits
Human IL-4 ELISpot kits
Pick the right ELISpot kit for Human IL-4. Our kits differ in what we put in the box and how you develop the signal – this page explains what those differences mean.
IL-4 ELISpot kits are also available for bovine, and mouse.
What differs between these kits
Whether a pre-coated plate is included
Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.
Which detection system
Available with Streptavidin-ALP, Streptavidin-HRP, or One-step ALP. With a streptavidin conjugate you add a second incubation after the biotinylated antibody, which amplifies the signal. A one-step conjugate arrives attached to the antibody, so developing takes a single incubation. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.
Supplied without a plate, streptavidin conjugate, enough for 4 plates
With a pre-coated white plate, one-step conjugate, 2 plates
With a pre-coated white plate, one-step conjugate, 10 plates
With a pre-coated white plate, streptavidin conjugate, 2 plates
With a pre-coated white plate, streptavidin conjugate, 10 plates
About IL-4
These Human IL-4 ELISpot kits target IL4 (Homo sapiens, Gene ID 3565).
Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.
| Also known as | Interleukin 4, IL-4, IL4, BCGF-1, BSF-1 |
| Produced by | T cell, Th2, Treg |
| Reactivity | Human and Non-human primates |
| Gene ID | 3565 |
Find the right kit
Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.
Pre-coated plate included
Or coat your own
Kit detection system
Which enzyme conjugate we supply
Next, choose whether you want a pre-coated plate.
Frequently bought together
What customers usually order alongside these kits.
Related reading
Guides and tutorials covering Human IL-4 and the ELISpot method.
