Catalog / ELISpot kits / IL-4 ELISpot kits
IL-4 ELISpot kits
We make ELISpot kits for IL-4 in 3 species. This page explains how they differ, so you can pick the one your experiment needs.
What differs between these kits
Which species they are for
Available for Bovine, Human, and Mouse. The species decides which antibody pair we supply, so it is the first thing to choose. Some kits are also validated on closely related species, so if you work in one we do not list here, check the reactivity on the product page.
Whether a pre-coated plate is included
Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.
Which detection system
Available with Streptavidin-ALP, Streptavidin-HRP, or One-step ALP. With a streptavidin conjugate you add a second incubation after the biotinylated antibody, which amplifies the signal. A one-step conjugate arrives attached to the antibody, so developing takes a single incubation. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.
About IL-4
Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.
Find the right IL-4 kit
Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.
Species
What you are measuring in
Pre-coated plate included
Or coat your own
Kit detection system
Which enzyme conjugate we supply
Start by choosing a species.
Frequently bought together
What customers usually order alongside these kits, whichever species they work in.
Related reading
Guides and tutorials covering IL-4 and the ELISpot method.
