ELISpot Plus: Human IL-17A (ALP)

ELISpot Plus: Human IL-17A (ALP)

3520-4APW-2

Publications: 31
Documents
Questions?
The easiest way to achieve reproducibility in ELISpot.

Components

Detection mAbMT504, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
Positive controlAnti-CD3 mAb (CD3-2)
PlatePre-coated ELISpot plate, white MSIP (mAb MT44.6)

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Delivery 4-9 business days

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PepPool: CEFTA (CD4), human


Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-17A using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Human IL-17A (ALP)
ApplicationELISpot
AnalyteIL-17A
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-17A.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
PBMC (125,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-17A was analyzed by ELISpot.
Datasheet/Protocol
SDS

Selecting the appropriate mAbs is key to effectively detecting human IL-17A/F. This mAb guide describes which combination of our mAbs should be used in ELISA and ELISpot assays.

 

Application guide: ELISA

Our human IL-17A/F ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Coating mAb: MT504, unconjugated
Detection mAb: MTF411, biotin

ELISA IL-17A/F

Application guide: ELISpot

Our human IL-17A/F ELISpot kits can be used to detect the important cytokine directly upon secretion by responding cells.

Coating mAb: MTF411, unconjugated
Detection mAb: MT504, biotin

IL-17A/F ELISpot

 

 

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot
An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IL-17A

Analyte description

Interleukin 17A (IL-17A) is a potent proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has also been shown to be produced by activated CD8 T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce the expression of cytokines, chemokines, and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production, and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer, and transplant rejection.

Alternative namesInterleukin 17A, IL-17A, IL17A, CTLA-8, CTLA8, IL-17, IL17, ILA17
Cell typeTh17
Gene ID3605
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FluoroSpot Plus: Human IFN-γ/IL-22/IL-17A