ELISpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ (ALP)

ELISpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ (ALP)

3420-4AST-P1-1

Publications: 21
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A straightforward setup for functional analysis of a specific scientific question.

Components

Detection mAb7-B6-1, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)
Peptide poolsSARS-CoV-2 (S1), scanning
SARS-CoV-2 (SNMO), human

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to SARS-CoV-2 (S1 scan+SNMO) peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus. The peptides are 15-mers overlapping with 11 amino acids, covering the S1 domain of the spike protein (amino acid 13-685). The pool is supplied in two vials: pool 1 SARS-CoV-2 S1 peptides 1-83 (83 peptides) and pool 2 SARS-CoV-2 S1 peptides 84-166 (83 peptides).

The SARS-CoV-2 SNMO defined peptide pool contains 47 synthetic peptides from the human SARS-CoV-2 virus. The peptides are derived from the spike, nucleoprotein, membrane protein, ORF3a and ORF7a.

Recommendation

Product details

ProductELISpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ (ALP)
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Human PBMC (300,000 cells/well) from a convalescent individual (B), previously confirmed COVID-19 positive with PCR, and an individual recovering from COVID-19 symptoms (A), were incubated overnight in the absence or presence of the SARS-CoV-2 defined peptide pool, SARS-CoV-2 S1 scanning pool vial 1, SARS-CoV-2 S1 scanning pool vial 2, SARS-CoV-2 S1 scanning pool vials 1+2 and anti-CD28 mAb. PHA was included as positive control. The number of cells secreting IFN-γ was analyzed by ELISpot.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458
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