FluoroSpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ/Granzyme B

FluoroSpot Path: SARS‑CoV-2 (S1scan+SNMO) Human IFN-γ/Granzyme B


Publications: 0
Paves your way to functional analysis of a specific research question.


PlatePre-coated FluoroSpot plate (mAbs 1-D1K and MT34B6)
Detection mAbsAnti-IFN-γ mAb (7-B6-1), BAM
Anti-Granzyme B (MT28), biotin
Fluorophore conjugatesAnti-BAM mAb, 490
Buffer/SolutionFluoroSpot enhancer
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)
Peptide poolsSARS-CoV-2 (S1), scanning
SARS-CoV-2 (SNMO), human

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This FluoroSpot Path kit is intended for the enumeration of cells secreting human IFN-γ and granzyme B in response to SARS-CoV-2 (S1 scan+SNMO) peptides using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus. The peptides are 15-mers overlapping with 11 amino acids, covering the S1 domain of the spike protein (amino acid 13-685). The pool is supplied in two vials: pool 1 SARS-CoV-2 S1 peptides 1-83 (83 peptides) and pool 2 SARS-CoV-2 S1 peptides 84-166 (83 peptides).

The SARS-CoV-2 SNMO defined peptide pool contains 47 synthetic peptides from the human SARS-CoV-2 virus. The peptides are derived from the spike, nucleoprotein, membrane protein, ORF3a and ORF7a.


Product details

ProductFluoroSpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ/Granzyme B
AnalyteIFN-γ, Granzyme B

This kit is based on matched pairs of mAbs specific for human IFN-γ and granzyme B.

Shipping and Storage


Shipped at ambient temperature.


Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
PBMC from a COVID-19 convalescent individual and PBMC from a healthy control were incubated 48 h in the absence or presence of the SARS-CoV-2 S1 scanning pool and SARS-CoV-2 S N M O defined peptide and anti-CD28 mAb. As a positive control, cells were stimulated with PHA + anti-C28 mAb. The number of cells secreting IFN-γ, Granzyme B and both analytes was analyzed by FluoroSpot using Mabtech IRIS.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.
Loading publications...


Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458

Granzyme B

Analyte description

Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.

Alternative namesGranzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell typeT cell, Tc, Th1, NK cell
Gene ID3002