FluoroSpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ/IL-2

FluoroSpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ/IL-2


Publications: 16
Paves your way to functional analysis of a specific research question.


PlatePre-coated FluoroSpot plate (mAbs 1-D1K and MT2A91/2C95)
Detection mAbsAnti-IFN-γ mAb (7-B6-1), BAM
Anti-IL-2 mAb (MT8G10), biotin
Fluorophore conjugatesAnti-BAM mAb, 490
Buffer/SolutionFluoroSpot enhancer
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)
Peptide poolsSARS-CoV-2 (S1), scanning
SARS-CoV-2 (SNMO), human

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Product specifications

Intended use

This FluoroSpot Path kit is intended for the enumeration of cells secreting human IFN-γ and IL-2 in response to SARS-CoV-2 (S1 scan+SNMO) peptides using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus. The peptides are 15-mers overlapping with 11 amino acids, covering the S1 domain of the spike protein (amino acid 13-685). The pool is supplied in two vials: pool 1 SARS-CoV-2 S1 peptides 1-83 (83 peptides) and pool 2 SARS-CoV-2 S1 peptides 84-166 (83 peptides).

The SARS-CoV-2 SNMO defined peptide pool contains 47 synthetic peptides from the human SARS-CoV-2 virus. The peptides are derived from the spike, nucleoprotein, membrane protein, ORF3a and ORF7a.


Product details

ProductFluoroSpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ/IL-2
AnalyteIFN-γ, IL-2

This kit is based on matched pairs of mAbs specific for human IFN-γ and IL-2.

Shipping and Storage


Shipped at ambient temperature.


Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Human PBMC from three COVID-19 convalescent individuals, previously confirmed SARS-CoV-2 positive with PCR, and PBMC from one individual collected before the SARS-CoV-2 pandemic were incubated overnight in the absence or presence of the SARS-CoV-2 S1 scanning pool and anti-CD28 mAb. The number of cells secreting IFN-γ, IL-2 and both analytes was analyzed by FluoroSpot using Mabtech IRIS.

Tutorial, Published November 24, 2022

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Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458


Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF, lymphokine
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID3558
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