SHOP

ELISpot Flex: Human Granzyme B (ALP)

PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1), and the number of cells secreting Granzyme B was analyzed by ELISpot.

ELISpot Flex: Human Granzyme B (ALP)

3486-2A

Special offer

You may add any of these complementary products at a reduced price.

PepPool: CEFRAS Global (CD8), human
  • Reactivity:
  • Application:
  • Plates:
PepPool: CEFRAS Global (CD4 and CD8), human
  • Reactivity:
  • Application:
  • Plates:

This offer is valid when purchasing ELISpot Flex: Human Granzyme B (ALP) or other qualifying products.

Publications: 62
Documents
Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT28
Detection mAbMT8610, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
1 / 6

ELISpot substrate: BCIP/NBT-plus for ALP

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human granzyme B using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human Granzyme B (ALP)
ApplicationELISpot
AnalyteGranzyme B
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human granzyme B.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.

PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1), and the number of cells secreting Granzyme B was analyzed by ELISpot.
PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1), and the number of cells secreting Granzyme B was analyzed by ELISpot.

Datasheet/ProtocolSDS

Featured product

New positive control peptide pools: CEFRAS Global

Discover the PepPool: CEFRAS Global family of peptide pools delivering superior T cell stimulation across diverse populations. With meticulously chosen epitopes from a number of common pathogens, it sets a new standard for ELISpot, FluoroSpot, and flow cytometry.

Tutorial

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.
Tutorial

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.
Tutorial

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.
Tutorial

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
Tutorial

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Loading publications...

Granzyme B

Analyte description

Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.

Alternative namesGranzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell typeT cell, Tc, Th1, NK cell
Gene ID3002

1 / 1

ELISpot Plus: Human Granzyme B (ALP)