Catalog / Human IL-21 ELISpot kits

Human IL-21 ELISpot kits

Pick the right ELISpot kit for Human IL-21. Our kits differ in what we put in the box and how you develop the signal – this page explains what those differences mean.

What differs between these kits

Whether a pre-coated plate is included

Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.

Which detection system

Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.

Supplied without a plate, streptavidin conjugate, enough for 4 plates

With a pre-coated white plate, streptavidin conjugate, 2 plates

With a pre-coated white plate, streptavidin conjugate, 10 plates

About IL-21

These Human IL-21 ELISpot kits target IL21 (Homo sapiens, Gene ID 59067).

Interleukin 21 (IL-21) is primarily secreted by CD4 T cells (particularly follicular T cells and Th17) and is an important regulator of immune responses. For Th17 cells, IL-21 is an autocrine regulator sustaining Th17 development. IL-21 also regulates the development of other T cells as well as B cells, NK cells, and dendritic cells. Areas of particular interest for research on IL-21 include autoimmune and inflammatory diseases, cancer research, and basic studies on, e.g., B cells and T cells, especially on Th17 cells.