FluoroSpot Plus: Human IFN-γ/IL-10/IL-5

FluoroSpot Plus: Human IFN-γ/IL-10/IL-5

FSP-010708-2

Publications: 7
Documents
Questions?
Ensure reproducibility when diving into a multiplex analysis.

Components

Detection mAbsAnti-IFN-γ mAb (7-B6-1), BAM
Anti-IL-10 mAb (12G8), biotin
Anti-IL-5 mAb (5A10), WASP
Fluorophore conjugatesAnti-BAM mAb, 490
SA-550
Anti-WASP mAb, 640
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)
Buffer/SolutionFluoroSpot enhancer
PlatePre-coated FluoroSpot plate (mAbs 1-D1K, 9D7, and TRFK5)

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
1 / 1

Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-γ, IL-10, and IL-5 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductFluoroSpot Plus: Human IFN-γ/IL-10/IL-5
ApplicationFluoroSpot
AnalyteIFN-γ, IL-10, IL-5
ReactivityHuman
Specificity

This kit is based on matched pairs of mAbs specific for human IFN-γ, IL-10, and IL-5.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
PBMC (250,000 cells/well) were incubated in the absence or presence of tetanus toxin and anti-CD28 monoclonal antibody for 48 hours and the number of cells secreting IFN-γ, IL-10 and/or IL-5 was analyzed by FluoroSpot. Double- and triple secreting cells were determined as spots having the same position (centre point) in an image overlay of IFN-γ, IL-10 and IL-5 images. Spot analysis was made with Mabtech IRIS

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.
Loading publications...

IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458

IL-10

Analyte description

Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.

Alternative namesInterleukin-10, IL-10, IL10, CSIF, GVHDS, IL-10A, TGIF
Cell typeT cell, Treg, Monocyte/MΦ, mDC
Gene ID3586

IL-5

Analyte description

Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells.  IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.

Alternative namesInterleukin-5, IL-5, IL5, BCGF-2, EDF, TRF
Cell typeT cell, Th2, NK cell
Gene ID3567