ELISpot Path: SARS‑CoV‑2 (S1scan​+​SNMO) Human Granzyme B

PBMC (250,000 cells/well) from two convalescent individuals, previously confirmed COVID-19 positive, were incubated 48 h in the absence or presence of the SARS-CoV-2 S1 scanning pool and SARS-CoV-2 S N M O defined peptide pool in the presence of anti-CD28 mAb. The number of cells secreting Granzyme B was analyzed by ELISpot and counted using MabtechIRIS.

ELISpot Path: SARS‑CoV‑2 (S1scan​+​SNMO) Human Granzyme B

3486-4APW-P1-1

$1,180

Publications: 1
Documents
Bulk or custom format
A straightforward setup for functional analysis of a specific scientific question.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT28)
Detection mAbMT8610, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
SubstrateBCIP/NBT-plus substrate for ELISpot
Peptide poolsSARS-CoV-2 (S1), scanning
SARS-CoV-2 (SNMO), human

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human granzyme B in response to SARS-CoV-2 (S1 scan+SNMO) peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus. The peptides are 15-mers overlapping with 11 amino acids, covering the S1 domain of the spike protein (amino acid 13-685). The pool is supplied in two vials: pool 1 SARS-CoV-2 S1 peptides 1-83 (83 peptides) and pool 2 SARS-CoV-2 S1 peptides 84-166 (83 peptides).

The SARS-CoV-2 SNMO defined peptide pool contains 47 synthetic peptides from the human SARS-CoV-2 virus. The peptides are derived from the spike, nucleoprotein, membrane protein, ORF3a and ORF7a.

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Product details

ProductELISpot Path: SARS-CoV-2 (S1scan+SNMO) Human Granzyme B
ApplicationELISpot
AnalyteGranzyme B
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human granzyme B.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
PBMC (250,000 cells/well) from two convalescent individuals, previously confirmed COVID-19 positive, were incubated 48 h in the absence or presence of the SARS-CoV-2 S1 scanning pool and SARS-CoV-2 S N M O defined peptide pool in the presence of anti-CD28 mAb. The number of cells secreting Granzyme B was analyzed by ELISpot and counted using MabtechIRIS.
PBMC (250,000 cells/well) from two convalescent individuals, previously confirmed COVID-19 positive, were incubated 48 h in the absence or presence of the SARS-CoV-2 S1 scanning pool and SARS-CoV-2 S N M O defined peptide pool in the presence of anti-CD28 mAb. The number of cells secreting Granzyme B was analyzed by ELISpot and counted using MabtechIRIS.
Datasheet/ProtocolSDS

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Granzyme B

Analyte description

Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.

Alternative namesGranzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell typeT cell, Tc, Th1, NK cell
Gene ID3002
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FluoroSpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-γ/Granzyme B