ELISpot Flex: Human IL‑27 (ALP)

Human IL-27 ELISpot. PBMC incubated in the presence or absence of LPS and recombinant human IFN-γ.

ELISpot Flex: Human IL‑27 (ALP)

3458-2A

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Bulk or custom format

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT27/361
Detection mAb	MT140, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot

In stock

Delivery 4-9 business days

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Complementary products

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ELISpot substrate: BCIP/NBT-plus for ALP

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-27 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Human IL-27 (ALP)
Application	ELISpot
Analyte	IL-27
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-27.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

PBMC (100,000 cells/well) were incubated for 22 h in the absence or presence of LPS and recombinant human IFN-γ and the number of cells secreting IL-27 was analyzed by ELISpot. Spot analysis was made with Mabtech IRIS.

Datasheet/Protocol SDS

Tutorial

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

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IL-27

Analyte description	The cytokine interleukin 27 (IL-27) is a member of the IL-12 family. IL-27 is a heterodimer composed of one EBI3 subunit and one p28 subunit. Monocytes, endothelial cells, dendritic cells, and T cells produce it. IL-27 has been described to have both pro-inflammatory and anti-inflammatory properties.
Alternative names	Interleukin 27, IL-27, IL27, IL-27AA, IL27p28, IL30, p28, IL-27B, IL27B, IL35B
Cell type	Monocyte/MΦ, mDC, NK cell
Gene ID	246778, 10148

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ELISpot Plus: Human IL-27 (ALP)