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ELISpot Plus: Human IL‑27 (ALP)

Human IL-27 ELISpot. PBMC incubated in the presence or absence of LPS and recombinant human IFN-γ.

ELISpot Plus: Human IL‑27 (ALP)

3458-4APW-2

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Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAbs MT27/361)
Detection mAbMT140, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
SubstrateBCIP/NBT-plus substrate for ELISpot

In stock

Delivery 4-9 business days

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Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-27 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Human IL-27 (ALP)
ApplicationELISpot
AnalyteIL-27
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-27.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.

Human IL-27 ELISpot. PBMC incubated in the presence or absence of LPS and recombinant human IFN-γ.
PBMC (100,000 cells/well) were incubated for 22 h in the absence or presence of LPS and recombinant human IFN-γ and the number of cells secreting IL-27 was analyzed by ELISpot. Spot analysis was made with Mabtech IRIS.

Datasheet/ProtocolSDS

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IL-27

Analyte description

The cytokine interleukin 27 (IL-27) is a member of the IL-12 family. IL-27 is a heterodimer composed of one EBI3 subunit and one p28 subunit. Monocytes, endothelial cells, dendritic cells, and T cells produce it. IL-27 has been described to have both pro-inflammatory and anti-inflammatory properties. 

Alternative namesInterleukin 27, IL-27, IL27, IL-27AA, IL27p28, IL30, p28, IL-27B, IL27B, IL35B
Cell typeMonocyte/MΦ, mDC, NK cell
Gene ID246778, 10148