Catalog / Monkey IP-10 ELISpot kits

Monkey IP-10 ELISpot kits

Pick the right ELISpot kit for Monkey IP-10. Our kits differ in what we put in the box and how you develop the signal – this page explains what those differences mean.

IP-10 ELISpot kits are also available for human.

What differs between these kits

Whether a pre-coated plate is included

Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.

Which detection system

Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.

Supplied without a plate, streptavidin conjugate, enough for 4 plates

Supplied without a plate, streptavidin conjugate, enough for 4 plates

With a pre-coated white plate, streptavidin conjugate, 2 plates

With a pre-coated white plate, streptavidin conjugate, 2 plates

With a pre-coated white plate, streptavidin conjugate, 10 plates

With a pre-coated white plate, streptavidin conjugate, 10 plates

About IP-10 (CXCL10)

These Monkey IP-10 ELISpot kits target CXCL10 (Homo sapiens, Gene ID 3627).

Interferon gamma-induced protein 10 (IP-10) secretion is induced by IFN-γ, TNF-α, and IL-1β. This proinflammatory cytokine is produced by monocytes, endothelial cells, and fibroblasts. The interferon gamma-induced protein 10 recruits lymphocytes to sites of infection and increases the expression of MHC class I molecules. In addition to chemotaxis, IP-10 is also involved in the differentiation and activation of peripheral immune cells, regulation of cell growth, apoptosis, and modulation of angiostatic effects. The binding of IP-10 (CXCL10) to the CXCR3 receptor causes the downstream activation of the phospholipase C-dependent pathway, an increase in intracellular calcium production, and actin reorganization. 

Also known asInterferon gamma-induced protein 10, IP-10, C-X-C motif chemokine ligand 10, CXCL10, small-inducible cytokine B10
Produced byT cell, Monocyte/MΦ
ReactivityNon-human primates
Gene ID3627

Find the right kit

Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.

Pre-coated plate included

Or coat your own

Kit detection system

Which enzyme conjugate we supply


Next, choose whether you want a pre-coated plate.

Frequently bought together

What customers usually order alongside these kits.

ELISpot substrate: BCIP/NBT-plus for ALP

Substrates

ELISpot substrate: BCIP/NBT-plus for ALP

Related reading

Guides and tutorials covering Monkey IP-10 and the ELISpot method.

Tutorials

Tutorial

2022-11-24

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.