Catalog / ELISpot kits / IP-10 ELISpot kits
IP-10 ELISpot kits
We make ELISpot kits for IP-10 in 2 species. This page explains how they differ, so you can pick the one your experiment needs.
What differs between these kits
Which species they are for
Available for Human and Monkey. The species decides which antibody pair we supply, so it is the first thing to choose. Some kits are also validated on closely related species, so if you work in one we do not list here, check the reactivity on the product page.
Whether a pre-coated plate is included
Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.
Which detection system
Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.
About IP-10 (CXCL10)
Interferon gamma-induced protein 10 (IP-10) secretion is induced by IFN-γ, TNF-α, and IL-1β. This proinflammatory cytokine is produced by monocytes, endothelial cells, and fibroblasts. The interferon gamma-induced protein 10 recruits lymphocytes to sites of infection and increases the expression of MHC class I molecules. In addition to chemotaxis, IP-10 is also involved in the differentiation and activation of peripheral immune cells, regulation of cell growth, apoptosis, and modulation of angiostatic effects. The binding of IP-10 (CXCL10) to the CXCR3 receptor causes the downstream activation of the phospholipase C-dependent pathway, an increase in intracellular calcium production, and actin reorganization.
Find the right IP-10 kit
Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.
Species
What you are measuring in
Pre-coated plate included
Or coat your own
Kit detection system
Which enzyme conjugate we supply
Start by choosing a species.
Frequently bought together
What customers usually order alongside these kits, whichever species they work in.
Related reading
Guides and tutorials covering IP-10 and the ELISpot method.
