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ELISpot Flex: Mouse TNF‑α (HRP)

ELISpot Flex: Mouse TNF‑α (HRP)

3511-2H

Publications: 20
Documents
Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT1C8/23C9
Detection mAbMT11B10, biotin
Enzyme conjugateStreptavidin-HRP enzyme conjugate

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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ELISpot substrate: TMB for HRP

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse TNF-α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Mouse TNF-α (HRP)
ApplicationELISpot
AnalyteTNF-α
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse TNF-α.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.

Datasheet/ProtocolSDS

Tutorial

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.
Tutorial

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.
Tutorial

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.
Tutorial

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
Tutorial

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF, TNFSF2, TNFalphaa, Tnfsf1a, Tnlg1f, Tnf
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID21926

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ELISpot Plus: Mouse TNF-α (ALP)