ELISpot Plus: Mouse TNF-α (ALP)

ELISpot Plus: Mouse TNF‑α (ALP)


Publications: 13
The easiest way to achieve reproducibility in ELISpot.


PlatePre-coated ELISpot plate, white MSIP (mAbs MT1C8/23C9)
Detection mAbMT11B10, biotin
Enzyme conjugateStreptavidin-ALP

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse TNF-α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples


Product details

ProductELISpot Plus: Mouse TNF-α (ALP)
SpecificityThis kit is based on a matched pair of mAbs specific for mouse TNF-α.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting TNF-alpha (TNF-α) was analyzed by ELISpot.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF, TNFSF2, TNFalphaa, Tnfsf1a, Tnlg1f, Tnf
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID21926
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