ELISpot Flex: Mouse IFN‑γ (HRP)

3321-2H

Publications: 1150

Documents

Bulk or custom format

Our most adaptable ELISpot format. Combine it with substrate, plate, antigen of choice, and Mabtech ASTOR 2 – a reader designed around your ELISpot workflow.

Components

Capture mAb	AN18
Detection mAb	R4-6A2, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate

In stock

Delivery 4-9 business days

Shipping $0

Frequently bought together

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ELISpot substrate: TMB for HRP

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Mouse IFN-γ (HRP)
Application	ELISpot
Analyte	IFN-γ
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs specific for mouse IFN-γ.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Datasheet/Protocol SDS

Tutorial

2022-11-24

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial

2022-11-10

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial

2022-11-07

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial

2022-10-14

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial

2022-10-14

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

Highlighted research

2020-05-20

Will ELISpot be key to the development of vaccines against SARS-CoV-2?

To study protective immune responses Mabtech mouse IFN-γ ELISpot was employed during preclinical vaccine research.

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IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, If2f, Ifg
Cell type	T cell, Tc, Th1, NK cell
Gene ID	15978

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ELISpot Plus: Mouse IFN-γ (HRP)