ELISpot Flex: Mouse IFN‑γ (ALP)

Mouse spleen cells (150,000 cells/well) were incubated overnight in the absence or presence of Concanavalin A (ConA) and the number of cells secreting IFN-γ was analyzed by ELISpot.

ELISpot Flex: Mouse IFN‑γ (ALP)

3321-2A

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Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbAN18
Detection mAbR4-6A2, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot

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Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Mouse IFN-γ (ALP)
ApplicationELISpot
AnalyteIFN-γ
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (150,000 cells/well) were incubated overnight in the absence or presence of Concanavalin A (ConA) and the number of cells secreting IFN-γ was analyzed by ELISpot.
Mouse spleen cells (150,000 cells/well) were incubated overnight in the absence or presence of Concanavalin A (ConA) and the number of cells secreting IFN-γ was analyzed by ELISpot.

Highlighted research
Published: October 11, 2024 | 8 minute read

Immunoassays for vaccine research

Explore the critical role of immunoassays like ELISpot and FluoroSpot in vaccine development, from evaluating immune responses to tracking vaccine efficacy and potency. These assays provide sensitive, single-cell insights for advancing research and development of viral, bacterial, and cancer vaccines.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, If2f, Ifg
Cell typeT cell, Tc, Th1, NK cell
Gene ID15978
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