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ELISpot Flex: Monkey IP‑10 (HRP)

PBMC from cynomolgus macaque (40,000 cells/well) were incubated for 20 h in the absence or presence of PHA and the number of cells secreting IP-10 (CXCL10) was analyzed by ELISpot.
PBMC from rhesus macaque (40,000 cells/well) were incubated for 20 h in the absence or presence of PHA and the number of cells secreting IP-10 (CXCL10) was analyzed by ELISpot.

ELISpot Flex: Monkey IP‑10 (HRP)

3401M-2H

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Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMTP11
Detection mAbMTP13, biotin
Enzyme conjugateStreptavidin-HRP enzyme conjugate

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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ELISpot plates, white

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting monkey IP-10 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Monkey IP-10 (HRP)
ApplicationELISpot
AnalyteIP-10 (CXCL10)
ReactivityNon-human primates
Specificity

This kit is based on a matched pair of mAbs enabling specific detection of IP-10 (CXCL10) from non-human primates (NHP).

Cross-reactivity

The mAbs react with human IP-10 and cross-react with IP-10 from NHP. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.

PBMC from cynomolgus macaque (40,000 cells/well) were incubated for 20 h in the absence or presence of PHA and the number of cells secreting IP-10 (CXCL10) was analyzed by ELISpot.
PBMC from cynomolgus macaque (40,000 cells/well) were incubated for 20 h in the absence or presence of PHA and the number of cells secreting IP-10 (CXCL10) was analyzed by ELISpot.
PBMC from cynomolgus macaque (40,000 cells/well) were incubated for 20 h in the absence or presence of PHA and the number of cells secreting IP-10 (CXCL10) was analyzed by ELISpot.
PBMC from rhesus macaque (40,000 cells/well) were incubated for 20 h in the absence or presence of PHA and the number of cells secreting IP-10 (CXCL10) was analyzed by ELISpot.

Datasheet/ProtocolSDS

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Human and monkey IP-10 ELISpot now available

IP-10, also called CXCL10 or Interferon-γ-induced Protein 10, got its first antibody pair.

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IP-10 (CXCL10)

Analyte description

Interferon gamma-induced protein 10 (IP-10) secretion is induced by IFN-γ, TNF-α, and IL-1β. This proinflammatory cytokine is produced by monocytes, endothelial cells, and fibroblasts. The interferon gamma-induced protein 10 recruits lymphocytes to sites of infection and increases the expression of MHC class I molecules. In addition to chemotaxis, IP-10 is also involved in the differentiation and activation of peripheral immune cells, regulation of cell growth, apoptosis, and modulation of angiostatic effects. The binding of IP-10 (CXCL10) to the CXCR3 receptor causes the downstream activation of the phospholipase C-dependent pathway, an increase in intracellular calcium production, and actin reorganization. 

Alternative namesInterferon gamma-induced protein 10, IP-10, C-X-C motif chemokine ligand 10, CXCL10, small-inducible cytokine B10
Cell typeT cell, Monocyte/MΦ
Gene ID3627