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ELISpot Flex: Human IL‑17F (ALP)

PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of PPD and the number of cells secreting IL-17F was analyzed by ELISpot.

ELISpot Flex: Human IL‑17F (ALP)

3522-2A

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Bulk or custom format
Our most adaptable ELISpot format. Combine it with substrate, plate, antigen of choice, and Mabtech ASTOR 2 – a reader designed around your ELISpot workflow.

Components

Capture mAbMTF1927
Detection mAbMTF912, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot

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Delivery 4-9 business days

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Frequently bought together

Frequently bought together
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ELISpot substrate: BCIP/NBT-plus for ALP

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-17F using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human IL-17F (ALP)
ApplicationELISpot
AnalyteIL-17F
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-17F.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.

PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of PPD and the number of cells secreting IL-17F was analyzed by ELISpot.
PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of PPD and the number of cells secreting IL-17F was analyzed by ELISpot.

Datasheet/ProtocolSDS

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IL-17F

Analyte description

Interleukin 17F (IL-17F) is a homodimer composed of two IL-17F subunits. IL17-F is a member of the IL-17A protein family. This proinflammatory cytokine is produced by activated Th17 (T helper 17) cells and certain cells of the innate immune system. Th17 cells play an important role in protection against bacteria and fungi. IL-17F contributes to the pathogenesis of inflammatory and autoimmune diseases, for example, psoriasis.

Alternative namesInterleukin 17F, IL-17F, IL17F, CANDF6, IL17A, ML-1, ML1
Cell typeT cell, Tc, Th17
Gene ID112744