Catalog / ELISpot kits / IL-5 ELISpot kits

IL-5 ELISpot kits

We make ELISpot kits for IL-5 in 6 species. This page explains how they differ, so you can pick the one your experiment needs.

What differs between these kits

Which species they are for

Available for Bovine, Goat, Human, Mouse, Porcine, and Sheep. The species decides which antibody pair we supply, so it is the first thing to choose. Some kits are also validated on closely related species, so if you work in one we do not list here, check the reactivity on the product page.

Whether a pre-coated plate is included

Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.

Which detection system

Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.

About IL-5

Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells.  IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.

Also known asInterleukin-5, IL-5, IL5, BCGF-2
Produced byT cell, Th2, NK cell
SpeciesBovine, Goat, Human, Mouse, Porcine, Sheep
Gene IDs
Bos taurus: 280825
Homo sapiens: 3567
Mus musculus: 16191
Sus scrofa (pig): 397409

Find the right IL-5 kit

Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.

Species

What you are measuring in

Pre-coated plate included

Or coat your own

Kit detection system

Which enzyme conjugate we supply


Start by choosing a species.


Frequently bought together

What customers usually order alongside these kits, whichever species they work in.

ELISpot substrate: BCIP/NBT-plus for ALP

Substrates

ELISpot substrate: BCIP/NBT-plus for ALP


Related reading

Guides and tutorials covering IL-5 and the ELISpot method.

Tutorials

Tutorial

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