Catalog / ELISpot kits / IgG1 ELISpot kits

IgG1 ELISpot kits

We make ELISpot kits for IgG1 in 2 species. This page explains how they differ, so you can pick the one your experiment needs.

What differs between these kits

Which species they are for

Available for Human and Mouse. The species decides which antibody pair we supply, so it is the first thing to choose. Some kits are also validated on closely related species, so if you work in one we do not list here, check the reactivity on the product page.

Which detection system

Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.

About IgG1

Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with the highest serum concentrations of IgG1, followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. IgG1 binds to Fc receptors with high affinity and may also activate the complement system.

Also known asImmunoglobulin G1, IgG1
Produced byB cell
SpeciesHuman, Mouse
Gene IDs
Homo sapiens: 3500
Mus musculus: 16017

Find the right IgG1 kit

Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.

Species

What you are measuring in

Kit detection system

Which enzyme conjugate we supply


Start by choosing a species.


Frequently bought together

What customers usually order alongside these kits, whichever species they work in.

ELISpot substrate: BCIP/NBT-plus for ALP

Substrates

ELISpot substrate: BCIP/NBT-plus for ALP


Related reading

Guides and tutorials covering IgG1 and the ELISpot method.

Tutorials

Tutorial

2022-11-24

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