Catalog / ELISpot kits / IFN-γ ELISpot kits
IFN-γ ELISpot kits
We make ELISpot kits for IFN-γ in 15 species. This page explains how they differ, so you can pick the one your experiment needs.
What differs between these kits
Which species they are for
Available for Bovine, Canine, Cat, Chicken, Cotton rat, Equine, Ferret, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, and Woodchuck. The species decides which antibody pair we supply, so it is the first thing to choose. Some kits are also validated on closely related species, so if you work in one we do not list here, check the reactivity on the product page.
Whether a pre-coated plate is included
Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.
Which plate we supply
Available as White, Clear, or Strip. Strip plates let you use part of a plate at a time, which suits smaller or staggered experiments, and a full plate costs less per well. You'll find exact plate specifications on each product page.
Which detection system
Available with Streptavidin-ALP, Streptavidin-HRP, One-step ALP, or One-step HRP. With a streptavidin conjugate you add a second incubation after the biotinylated antibody, which amplifies the signal. A one-step conjugate arrives attached to the antibody, so developing takes a single incubation. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.
About IFN-γ
Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
| Also known as | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg |
| Produced by | T cell, Tc, Th1, NK cell |
| Species | Bovine, Canine, Cat, Chicken, Cotton rat, Equine, Ferret, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Woodchuck |
| Gene IDs | Bos taurus: 281237 Canis lupus familiaris: 403801 Equus caballus: 100034181 Felis catus: 493965 Gallus gallus: 396054 Homo sapiens: 3458 Marmota monax: 124083955 Mesocricetus auratus: 101825431 Mus musculus: 15978 Mustela putorius furo: 101693741 Oryctolagus cuniculus: 100008602 Ovis aries: 443396 Rattus norvegicus: 25712 Sus scrofa: 396991 |
Find the right IFN-γ kit
Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.
Species
What you are measuring in
Pre-coated plate included
Or coat your own
Plate type
Which plate we supply
Kit detection system
Which enzyme conjugate we supply
Start by choosing a species.
Frequently bought together
What customers usually order alongside these kits, whichever species they work in.
Related reading
Guides and tutorials covering IFN-γ and the ELISpot method.
