Catalog / Human GM-CSF ELISpot kits

Human GM-CSF ELISpot kits

Pick the right ELISpot kit for Human GM-CSF. Our kits differ in what we put in the box and how you develop the signal – this page explains what those differences mean.

What differs between these kits

Whether a pre-coated plate is included

Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.

Which detection system

Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.

Supplied without a plate, streptavidin conjugate, enough for 4 plates

With a pre-coated white plate, streptavidin conjugate, 2 plates

With a pre-coated white plate, streptavidin conjugate, 10 plates

About GM-CSF

These Human GM-CSF ELISpot kits target CSF2 (Homo sapiens, Gene ID 1437).

Granulocyte macrophage-colony stimulating factor (GM-CSF) can be secreted by T cells, macrophages, endothelial cells, and fibroblasts. GM-CSF stimulates the survival and functional activities of myeloid, that is, monocytes, macrophages, DCs, neutrophils, and eosinophils. It also stimulates the differentiation and proliferation of hematological progenitors.