Catalog / Human EBI3 ELISpot kits
Human EBI3 ELISpot kits
Pick the right ELISpot kit for Human EBI3. Our kits differ in what we put in the box and how you develop the signal – this page explains what those differences mean.
What differs between these kits
Whether a pre-coated plate is included
Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.
Which detection system
Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.
Supplied without a plate, streptavidin conjugate, enough for 4 plates
With a pre-coated white plate, streptavidin conjugate, 2 plates
With a pre-coated white plate, streptavidin conjugate, 10 plates
About EBI3
These Human EBI3 ELISpot kits target EBI3 (Homo sapiens, Gene ID 10148).
EBI3 is a protein subunit that forms heterodimers with other proteins in the IL-12 family. IL-27 is a heterodimer composed of the subunits EBI3 and p28. IL-35 is a heterodimer composed of the subunits EBI3 and p35. IL-39 is a heterodimer consisting of the subunits EBI3 and p19. Native IL-39 has not yet been detected in humans. The EBI3 has ca. 30% sequence similarity to IL-12 p40.
| Also known as | Epstein–Barr virus-induced gene3, EBI3, Interleukin-27 subunit beta, IL-27B, IL27B, IL35B |
| Produced by | Monocyte/MΦ, mDC |
| Reactivity | Human |
| Gene ID | 10148 |
Find the right kit
Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.
Pre-coated plate included
Or coat your own
Kit detection system
Which enzyme conjugate we supply
Next, choose whether you want a pre-coated plate.
Frequently bought together
What customers usually order alongside these kits.
Related reading
Guides and tutorials covering Human EBI3 and the ELISpot method.
