FluoroSpot Plus: Human IL‑2 (550)

Human IL-2 FluoroSpot. PBMC were incubated overnight in the absence or presence of anti-CD3 antibody. Analysis was made with Mabtech IRIS.

FluoroSpot Plus: Human IL‑2 (550)

FSP-02B-2

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This offer is valid when purchasing FluoroSpot Plus: Human IL-2 (550) or other qualifying products.

Publications: 41
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Ensure reproducibility when diving into a multiplex analysis.

Components

PlatePre-coated FluoroSpot plate (mAbs MT2A91/2C95)
Detection mAbAnti-IL-2 mAb (MT8G10), biotin
Fluorophore conjugateSA-550
Buffer/SolutionFluoroSpot enhancer
Positive controlAnti-CD3 mAb (CD3-2)
Co-stimulusAnti-CD28 mAb (CD28-A)

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Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IL-2 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductFluoroSpot Plus: Human IL-2 (550)
ApplicationFluoroSpot
AnalyteIL-2
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-2.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, fluorophore conjugate, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.

Human IL-2 FluoroSpot. PBMC were incubated overnight in the absence or presence of anti-CD3 antibody. Analysis was made with Mabtech IRIS.
PBMC (50,000 cells/well) were incubated overnight in the absence or presence of anti-CD3 mAb (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-2 was analyzed by FluoroSpot. Spot analysis was made with Mabtech IRIS.

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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF, lymphokine
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID3558

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