FluoroSpot Plus: Human IFN‑γ (550)
FluoroSpot Plus: Human IFN‑γ (550)
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You may add any of these complementary products at a reduced price.
PepPool: CEFRAS Global (CD8), human
- Reactivity:
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$300
$240PepPool: CEFRAS Global (CD4), human
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$245
$196PepPool: CEFRAS Global (CD4 and CD8), human
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$545
$436This offer is valid when purchasing FluoroSpot Plus: Human IFN-γ (550) or other qualifying products.
Publications: 113
Documents
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Ensure reproducibility when diving into a multiplex analysis.
Components
| Plate | Pre-coated FluoroSpot plate (mAb 1-D1K) |
| Detection mAb | Anti-IFN-γ mAb (7-B6-1), biotin |
| Fluorophore conjugate | SA-550 |
| Buffer/Solution | FluoroSpot enhancer |
| Positive control | Anti-CD3 mAb (CD3-2) |
| Co-stimulus | Anti-CD28 mAb (CD28-A) |
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Human PBMC were incubated for 24 hours in the FluoroSpot plate in the absence (250,000 cells/well) or presence (50,000) of anti-CD3 and anti-CD28 antibody. IFN-γ spots were analyzed with Mabtech IRIS.
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IFN-γ
| Analyte description | Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. |
| Alternative names | Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69 |
| Cell type | T cell, Tc, Th1, NK cell |
| Gene ID | 3458 |
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