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ELISpot Plus: Human EBI3 (ALP)

PBMC were incubated for 20 h in the presence (30,000 cells/well) or absence (100,000 cells/well) LPS and recombinant human IFN-γ and the number of cells secreting EBI3 was analyzed by ELISpot.

ELISpot Plus: Human EBI3 (ALP)

3459-4APW-2

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Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT23A14)
Detection mAbMT140, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
SubstrateBCIP/NBT-plus substrate for ELISpot

In stock

Delivery 4-9 business days

Shipping $0

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human EBI3 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

EBI3
Assays based on antibodies to EBI3 will detect both IL-27 and IL-35."3 will detect both IL-27 and IL-35.

Recommendation

Product details

ProductELISpot Plus: Human EBI3 (ALP)
ApplicationELISpot
AnalyteEBI3
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human EBI3 subunit of IL-27 and IL-35. The assay will detect both IL-27 and IL-35.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.

PBMC were incubated for 20 h in the presence (30,000 cells/well) or absence (100,000 cells/well) LPS and recombinant human IFN-γ and the number of cells secreting EBI3 was analyzed by ELISpot.
PBMC were incubated for 20 h in the presence (30,000 cells/well) or absence (100,000 cells/well) LPS and recombinant human IFN-γ and the number of cells secreting EBI3 was analyzed by ELISpot.

Datasheet/ProtocolSDS

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EBI3

Analyte description

EBI3 is a protein subunit that forms heterodimers with other proteins in the IL-12 family. IL-27 is a heterodimer composed of the subunits EBI3 and p28. IL-35 is a heterodimer composed of the subunits EBI3 and p35. IL-39 is a heterodimer consisting of the subunits EBI3 and p19. Native IL-39 has not yet been detected in humans. The EBI3 has ca. 30% sequence similarity to IL-12 p40.

Alternative namesEpstein–Barr virus-induced gene3, EBI3, Interleukin-27 subunit beta, IL-27B, IL27B, IL35B
Cell typeMonocyte/MΦ, mDC
Gene ID10148