ELISpot Flex: Rat IL‑22 (ALP)

3275-2A

Publications: 0

Documents

Bulk or custom format

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT230
Detection mAb	MT231, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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ELISpot substrate: BCIP/NBT-plus for ALP

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting rat IL-22 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Rat IL-22 (ALP)
Application	ELISpot
Analyte	IL-22
Reactivity	Rat
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of rat IL-22.
Cross-reactivity	The mAbs react with mouse IL-22 and cross-react with IL-22 from rat. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Datasheet/Protocol SDS

Tutorial

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

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IL-22

Analyte description	The cytokine interleukin 22 (IL-22) is mainly produced by activated CD4 T helper 17 (Th17) and T helper 22 (Th22) cells. The IL-22 receptor is expressed on non-immune cells, particularly epithelial cells and keratinocytes, and IL-22 promotes innate immune responses versus bacterial infections in these target cells. In addition, Th22 cells have been shown to play a role in the pathophysiology of several human skin diseases.
Alternative names	Interleukin-22, IL-22, IL22, IL-22a, ILTIFa, If2b1, Iltif
Cell type	T cell, Th1, Th17, Tfh
Gene ID	50929